Currently, the core lab is equipped with Illumina HiSeq 4000, HiSeq 2500, MiSeq, and Life Technologies Ion Torrent PGM. The core offers the following sequencing services:

  • Sequencing library construction and multiplexing / pooling of libraries
  • Sample and library quality control (qPCR, bioanalzyer, quantitation)
  • HiSeq 2500 and HiSeq4000
    • Single and Paired End Sequencing with read lengths of 50 cycles, 125 cycles, and 150 cycles
  • MiSeq
    • Single Read sequencing with read lengths of 50 cycles and 125 cycles
    • Paired End Sequencing with read lengths of 75 cycles, 150 cycles, 250 cycles, and 300 cycles
  • Ion PGM sequencing (200 to 400 base reads)

For recommendations about which type of run best suits your needs, please contact us.

Library Construction

The Core Lab offers a variety of library preparation services including: Total RNA, stranded mRNA, small RNA, ribo zero RNA, Genomic DNA, Chip DNA, Exome DNA, and target gene panels. The core can work with you to identify a library construction method that fits your needs. All RNA, and DNA samples submitted to the core facility go through QC prior to library construction.

A Note about Turnaround Time

We strive to meet a two to three week turnaround time for library construction and sequencing of your samples. If submitting ready to sequence libraries turnaround time is one to two weeks. Our sequencing queue is fluid and filled on a first come first served basis. We consistently sell individual sequencing lanes and rapidly fill flow cells for sequencing. Priority is given to UCCC/CCTSI members, and clients with full flow cells.

Typical Run Times:

  • MiSeq
  • Single Read - 1 day Paired End Reads - 2 days
  • HiSeq2500
  • Single Read 3- 4 days Paired End Read 6-7 days
  • HiSeq4000
  • Single Read 1-2 days Paired End Read 3-4 days