Genomics and Microarray Core's Platforms

illumina MiSeq
The Illumina MiSeq System combines proven sequencing by synthesis (SBS) reversible terminator chemistry technology with a workflow that enables cluster generation, sequencing, and base calling in 5 ~ 65 hours runtime. It uses a single flowcell single lane format. With the v3 reagents, it is capable to deliver up to 15 Gb information with 25 million sequencing reads and 2x300 bp read lengths. Sample prep kits are optimized for a variety of applications, such as small genome, amplicons, targeted gene panel sequencing. See more detail on Illumina website.

illumina HiSeq 2000/2500
The Illumina HiSeq2500 System is a powerful and efficient ultra-high-throughput sequencing system that supports the broadest range of applications and study sizes. The regular high output run mode, utilize two flowcell with each flowcell consisting of 8 lanes. With the newest chemistry (v4), the high output run mode is capable to deliver up to 1 Tb information per run with 2 billion sequencing reads and 2x125 bp read lengths. The rapid run mode, which utilize one flowcell with 2 lanes per run format, is capable to deliver up to 180 Gb information per run with 300 million sequencing reads and 2x150 bp read lengths in 40 hours runtime. See more detail on Illumina website.

illumina iScan
The iScan system utilizes BeadArray microarray technology for a broad range of DNA and RNA analysis applications. Each bead on Illumina arrays is covered with hundreds of thousands of copies of specific oligos that act as the capture sequences in one of our array-based assays. iScan systems possess the advanced laser and optics components capable of handling even the highest density multi-sample arrays, producing high-quality data with rapid turnaround times. See more details on Illumina website.

Affymetrix GeneChip Scanner 3000 7G System
The scanner is a 16-bit resolution data acquisition confocal scanner which measures fluorescence intensity emitted by the labeled cRNA/cDNA bound to the probe arrays. It uses a high-quality, solid state 532nm Green Laser and the optics are designed to condition the laser beam to deliver an excitation spot size of 3.5 μm. See more details on the Affymetrix website

Affymetrix GeneTitan System
GeneTitan system supports gene expression for human, mouse and rat samples on 16-, 24-, 96-array layouts and genotyping studies for human samples on 96- and 384-array layouts. See more details on the Affymetrix website.

Ion Torrent PGM
The Ion Torrent PGM System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data. Direct, real time sequencing detection provides sequencing results typically in less than 3 hours. The Ion PGM™ System supports up to 400–base reads. See more details on the Life Technologies website

Agilent Microarray SureScan
The SureScan Microarray Scanner is a laser induced fluorescence scanner designed to read microarrays printed on a standard 1 inch x 3 inch glass slides. It measures the fluorescence intensity of two dyes simultaneously from labeled sample nucleic acid bound to microarrays. See more details on the Agilent website.

2100 Agilent Bioanalyzer
The Agilent 2100 electrophoresis bioanalyzer is a microfluidics-based platform for the analysis of RNA, DNA and protein. It delivers high quality digital data using minimal sample. See more details on the Agilent website.

Covaris S220 Ultrasonicator
Covaris S220 Ultrasonicator utilizes an Adaptive Focused Acoustic (AFA) technology to delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. When operated at higher intensity levels, the instrument can create a tunable shock wave environment with subsequent shear jet forces which has been demonstrated to be ideal for tissue disruption and DNA fragmentation applications. See more details on the Covaris website.

Agilent 2200 Tape Station
The Agilent 2200 TapeStation system is a tape-based platform for RNA, DNA quality control. It provides simpler, faster and more reproducible data through minimal manual intervention using the pre-packaged assay ScreenTape. See more details on the Agilent website.

SOMAscan Assay
SOMAscan™ assay is a new proteomics research tool for life science research, discoveries of protein biomarkers for diagnostics and drug development. The current version of the assay is capable of measuring 1129 human proteins using 50 ul of plasma or serum samples. The protein quantification is fulfilled by SOMAmer (Slow Off-rate Modified Aptamer) reagents, which has unique nucleotide sequences recognizable by specific DNA hybridization probes and are also chemically modified to form three-dimensional structure to provide protein-binding specificity. The SOMAscan assay measures native proteins in complex matrices by transforming each individual protein concentration into a corresponding SOMAmer reagent concentration. The SOMAmer reagent concentration is then quantified by DNA microarray. For more information, please see SOMAscan™ assay.